

Software Version	3.16.10



Experiment File Path:	C:\Users\bnext\Documents\Biotek\experiments\20250613-cytation3-pure-timecourse-gfp-EXPERIMENT.xpt
Protocol File Path:	C:\Users\bnext\Documents\Biotek\protocols\cytation3-pure-timecourse-gfp.prt



Plate Number	Plate 1
Date	6/13/2025
Time	1:18:05 PM
Reader Type:	Cytation3
Reader Serial Number:	130723A
Reading Type	Reader

Procedure Details

Plate Type	Greiner 384 SV NoBind AutoMap (Use plate lid)
Well Selection	Runtime
Eject plate on completion	
Set Temperature	Setpoint 37C, Gradient 0 C
Start Kinetic	Runtime 6:00:00 (HH:MM:SS), Interval 0:05:00, 73 Reads
    Read	GFP-F-G35
	Fluorescence Endpoint
	Full Plate
	Filter Set 1 (2)
	    Excitation: 485/20,  Emission: 528/20
	    Mirror: Top 510 nm,  Gain: 35
	Light Source: Xenon Flash,  Lamp Energy: High
	Read Speed: Normal,  Delay: 100 msec,  Measurements/Data Point: 10
	Read Height: 11.5 mm
    Read	GFP-M-G100
	Fluorescence Endpoint
	Full Plate
	Filter Set 1
	    Excitation: 485,  Emission: 528
	    Optics: Top,  Gain: 75
	Light Source: Xenon Flash,  Lamp Energy: High
	Read Speed: Normal,  Delay: 100 msec,  Measurements/Data Point: 10
	Read Height: 10.75 mm
End Kinetic	
Set Temperature	Incubator off

Layout
	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24
A																									Well ID
B																									Well ID
C																									Well ID
D																									Well ID
E																									Well ID
F																									Well ID
G																									Well ID
H																									Well ID
I	SPL1			SPL1			SPL1																		Well ID
J																									Well ID
K	SPL2			SPL2			SPL2																		Well ID
L																									Well ID
M	SPL3			SPL3			SPL3																		Well ID
N																									Well ID
O	SPL4			SPL4			SPL4																		Well ID
P																									Well ID

GFP-F-G35:485/20,528/20

Time	T GFP-F-G35:485/20,528/20	I1	I4	I7	K1	K4	K7	M1	M4	M7	O1	O4	O7
0:00:00	29.9	43	45	47	43	44	44	43	42	42	43	43	42
0:05:00	33.4	43	45	47	44	44	45	44	43	42	42	43	42
0:10:00	36.2	44	47	49	53	54	55	54	53	52	50	51	49
0:15:00	37.0	50	54	58	92	94	98	97	96	98	85	91	87
0:20:00	37.0	60	64	71	174	183	193	186	191	195	157	173	165
0:25:00	36.9	70	72	83	378	412	445	393	427	454	293	332	319
0:30:00	36.9	84	85	101	1144	1285	1410	1132	1318	1434	717	824	798
0:35:00	37.0	107	103	134	3240	3579	3866	3160	3643	3892	1964	2213	2141
0:40:00	37.0	145	131	187	6872	7367	7792	6793	7509	7785	4480	4874	4685
0:45:00	37.0	201	172	262	11282	11818	12246	11402	12080	12256	8106	8627	8213
0:50:00	37.0	275	223	360	15668	16088	16587	16157	16625	16575	12303	12852	12101
0:55:00	37.0	365	285	475	19633	19957	20321	20524	20746	20408	16435	16921	15926
1:00:00	37.0	466	355	602	23013	23148	23543	24247	24111	23613	20145	20570	19348
1:05:00	37.0	573	428	739	25807	26017	26211	27413	27030	26310	23258	23707	22238
1:10:00	37.0	688	509	884	28351	28232	28562	30043	29327	28496	25867	26200	24471
1:15:00	36.9	804	592	1034	30254	30072	30454	32254	31319	30381	27952	28226	26374
1:20:00	37.0	926	680	1197	31949	31814	32058	34073	32919	31884	29579	29819	27785
1:25:00	37.0	1052	770	1365	33423	33291	33543	35643	34403	33175	31111	31317	28952
1:30:00	37.0	1183	866	1548	34698	34386	34657	36947	35523	34176	32286	32293	29929
1:35:00	37.0	1323	972	1743	35759	35622	35726	38024	36557	35131	33160	33439	30741
1:40:00	37.0	1473	1081	1956	36769	36374	36633	38847	37235	35904	33876	34024	31440
1:45:00	37.0	1621	1196	2182	37509	37297	37307	39541	37939	36447	34531	34689	31889
1:50:00	37.0	1788	1321	2429	38159	37862	38024	40161	38425	36931	35049	34980	32279
1:55:00	37.0	1955	1458	2692	38735	38267	38467	40775	38897	37479	35510	35521	32804
2:00:00	37.0	2143	1603	2968	39217	38963	38923	41179	39347	37716	35729	35827	32992
2:05:00	37.0	2335	1753	3247	39488	39217	39334	41496	39624	37927	36003	36153	33316
2:10:00	37.0	2535	1920	3534	39821	39604	39539	41848	39935	38210	36281	36314	33492
2:15:00	37.0	2744	2087	3828	40220	39885	39817	42046	40391	38428	36670	36671	33586
2:20:00	37.0	2950	2250	4115	40409	40051	40117	42364	40396	38477	36831	36923	33792
2:25:00	37.0	3157	2433	4394	40661	40328	40282	42468	40389	38697	36990	37070	33907
2:30:00	37.0	3363	2590	4667	40861	40515	40441	42738	40786	38975	37185	37301	34065
2:35:00	37.0	3550	2769	4915	41027	40550	40621	42819	40809	39022	37277	37293	34258
2:40:00	37.0	3749	2920	5144	41075	40719	40719	43044	40964	39173	37373	37450	34333
2:45:00	37.0	3929	3079	5382	41217	40880	40851	43150	41101	39277	37490	37567	34390
2:50:00	37.0	4105	3234	5587	41340	40938	40974	43276	41328	39304	37663	37721	34542
2:55:00	37.0	4272	3386	5783	41562	41065	41034	43387	41250	39407	37804	37792	34571
3:00:00	37.0	4431	3525	5975	41632	41195	41092	43614	41415	39480	37733	37938	34693
3:05:00	37.0	4568	3645	6124	41599	41200	41280	43591	41479	39627	37895	37964	34784
3:10:00	37.0	4722	3766	6297	41666	41248	41274	43726	41621	39639	38030	38099	34913
3:15:00	37.0	4853	3894	6435	41952	41445	41523	43881	41817	39779	38158	38274	34919
3:20:00	37.0	4976	4012	6584	41917	41420	41452	43803	41677	39742	38019	38203	34935
3:25:00	37.0	5104	4117	6711	41880	41608	41496	43931	41894	39749	38160	38292	34964
3:30:00	37.0	5215	4234	6844	42054	41589	41501	44045	41733	39820	38187	38327	34997
3:35:00	37.0	5317	4328	6956	41989	41497	41543	43982	41908	39870	38272	38504	35002
3:40:00	37.0	5431	4440	7055	42140	41654	41476	44122	41854	39912	38181	38490	35024
3:45:00	37.0	5526	4512	7166	42095	41631	41590	44155	42016	39977	38318	38668	35134
3:50:00	37.0	5614	4625	7277	42285	41795	41647	44283	41943	39983	38226	38855	35184
3:55:00	37.0	5725	4699	7382	42199	41751	41687	44290	42082	39909	38347	38732	35208
4:00:00	37.0	5794	4782	7448	42179	41797	41775	44329	42051	39984	38381	38731	35135
4:05:00	37.0	5870	4855	7561	42161	41924	41818	44438	42188	39909	38374	38883	35298
4:10:00	37.0	5959	4937	7618	42332	41778	41783	44398	42142	40017	38526	38660	35183
4:15:00	37.0	6042	5000	7706	42301	41971	41805	44571	42235	40099	38515	38901	35349
4:20:00	37.0	6127	5094	7776	42266	41851	41785	44502	42117	40117	38480	38870	35337
4:25:00	37.0	6189	5149	7855	42324	41826	41818	44584	42104	40153	38512	38816	35397
4:30:00	37.0	6271	5214	7932	42492	41881	41896	44634	42432	40184	38626	39025	35450
4:35:00	37.0	6338	5290	8018	42425	41878	41996	44633	42279	40105	38546	39052	35430
4:40:00	37.0	6411	5342	8053	42459	42003	41933	44667	42356	40211	38680	38953	35424
4:45:00	37.0	6469	5428	8113	42509	41937	41938	44711	42275	40246	38603	38907	35493
4:50:00	37.0	6509	5470	8178	42454	41945	41886	44692	42270	40153	38619	38996	35444
4:55:00	37.1	6571	5533	8234	42522	42037	41912	44775	42375	40104	38695	39027	35521
5:00:00	36.9	6650	5583	8272	42562	42047	41938	44796	42250	40236	38607	39086	35494
5:05:00	37.0	6693	5631	8327	42467	41866	41947	44853	42275	40312	38697	38930	35577
5:10:00	37.0	6732	5676	8371	42479	41995	41982	44876	42401	40313	38821	39192	35561
5:15:00	37.0	6789	5727	8416	42399	41968	42026	44755	42324	40313	38711	39087	35541
5:20:00	36.9	6851	5791	8471	42414	42015	42006	44998	42503	40175	38773	39239	35539
5:25:00	37.0	6908	5833	8506	42678	42000	41969	44923	42451	40225	38707	39168	35616
5:30:00	37.0	6961	5891	8557	42424	42024	42005	44918	42436	40273	38837	39250	35608
5:35:00	37.0	6999	5930	8578	42536	42059	42031	44926	42372	40286	38812	39174	35628
5:40:00	37.0	7052	5947	8602	42418	41834	41920	44881	42536	40260	38716	39041	35526
5:45:00	37.0	7065	5984	8629	42421	41955	41923	44922	42303	40143	38693	39308	35476
5:50:00	36.9	7096	6032	8677	42320	41933	41907	44829	42325	40156	38642	39153	35560
5:55:00	37.0	7147	6079	8706	42470	42049	41975	45063	42387	40222	38722	39327	35586
6:00:00	37.0	7183	6123	8738	42488	42056	41930	45092	42312	40292	38723	39139	35685

GFP-M-G100:485,528

Time	T GFP-M-G100:485,528	I1	I4	I7	K1	K4	K7	M1	M4	M7	O1	O4	O7
0:00:23	30.2	11	23	20	16	12	13	11	11	19	16	12	11
0:05:23	33.6	12	12	7	9	12	14	7	19	9	12	13	16
0:10:23	36.3	18	14	16	18	17	16	10	14	13	16	10	8
0:15:23	37.0	17	17	18	25	37	34	33	34	32	27	16	27
0:20:23	37.0	25	26	22	61	63	67	59	58	67	48	54	56
0:25:23	37.0	21	23	21	130	133	162	119	148	163	109	108	107
0:30:23	36.9	19	25	28	405	454	505	367	465	534	258	279	282
0:35:23	37.0	27	29	42	1151	1264	1371	1047	1286	1422	713	764	754
0:40:23	37.0	47	40	59	2434	2595	2788	2210	2657	2848	1608	1656	1661
0:45:23	37.0	73	56	77	4015	4165	4393	3694	4281	4479	2926	2887	2887
0:50:23	37.0	79	73	108	5674	5815	5995	5364	5999	6122	4475	4352	4239
0:55:23	37.0	114	88	145	7169	7266	7409	6896	7536	7610	6010	5861	5665
1:00:23	37.0	146	106	182	8559	8598	8705	8260	8940	8898	7469	7197	6943
1:05:23	36.9	172	138	229	9722	9713	9792	9500	10141	10053	8712	8357	8028
1:10:23	37.0	217	164	270	10706	10710	10809	10518	11113	11023	9764	9286	8980
1:15:23	37.0	253	183	314	11561	11596	11607	11373	12018	11772	10684	10143	9694
1:20:23	37.0	273	210	358	12307	12312	12284	12108	12746	12557	11433	10841	10340
1:25:23	37.0	320	247	414	13044	13055	12995	12850	13399	13102	12030	11366	10835
1:30:23	37.0	362	270	472	13572	13578	13507	13371	13979	13617	12553	11768	11263
1:35:23	37.0	408	301	533	14086	14053	14037	13852	14466	14026	12959	12165	11618
1:40:23	37.0	450	339	592	14581	14482	14478	14203	14832	14429	13332	12462	11779
1:45:23	37.0	518	367	657	14920	14948	14870	14576	15104	14703	13574	12763	12097
1:50:23	37.0	550	407	731	15253	15214	15132	14767	15298	14934	13778	12987	12214
1:55:23	37.0	606	456	824	15544	15443	15395	15062	15642	15174	14019	13141	12380
2:00:23	37.0	667	499	896	15754	15679	15560	15256	15842	15266	14248	13269	12535
2:05:23	37.0	731	548	983	15960	15913	15786	15394	15994	15472	14378	13387	12726
2:10:23	37.1	770	608	1072	16034	16088	15894	15577	16133	15594	14529	13554	12815
2:15:23	37.0	838	648	1151	16224	16179	16017	15647	16289	15633	14629	13597	12831
2:20:23	37.0	905	712	1252	16401	16427	16116	15783	16352	15764	14686	13714	12925
2:25:23	37.0	986	773	1340	16454	16456	16251	15914	16470	15870	14826	13751	13060
2:30:23	37.0	1058	819	1396	16580	16556	16315	16037	16559	15938	14924	13889	13147
2:35:23	37.0	1102	877	1500	16731	16662	16365	16071	16621	15995	14943	13892	13177
2:40:23	37.0	1179	930	1563	16774	16730	16481	16173	16617	16109	14971	13910	13246
2:45:23	36.9	1227	967	1618	16795	16753	16501	16168	16732	16110	15103	14011	13298
2:50:23	37.0	1268	1025	1709	16895	16850	16518	16251	16767	16210	15189	14167	13301
2:55:23	37.0	1340	1064	1757	16905	16853	16658	16338	16843	16228	15160	14094	13356
3:00:23	37.0	1384	1100	1814	16989	16944	16726	16245	16937	16255	15275	14170	13359
3:05:23	37.0	1448	1137	1875	17013	16966	16740	16419	16920	16360	15278	14234	13415
3:10:23	37.0	1463	1188	1925	17105	17051	16845	16527	16991	16302	15270	14320	13442
3:15:23	37.0	1520	1232	1991	17181	17024	16858	16460	16985	16403	15385	14312	13424
3:20:23	37.0	1560	1278	2009	17107	17155	16855	16459	16974	16431	15312	14342	13501
3:25:23	37.0	1585	1314	2059	17182	17112	16944	16516	17055	16448	15453	14340	13487
3:30:23	37.0	1618	1324	2093	17239	17133	16874	16640	17059	16486	15430	14398	13520
3:35:23	37.0	1664	1379	2132	17283	17214	17007	16641	17155	16492	15407	14408	13549
3:40:23	37.0	1693	1392	2164	17277	17219	16990	16632	17077	16600	15510	14386	13564
3:45:23	37.0	1730	1439	2220	17365	17260	16980	16704	17167	16521	15544	14454	13594
3:50:23	37.0	1745	1450	2231	17285	17291	17025	16736	17153	16554	15554	14482	13673
3:55:23	37.0	1795	1466	2249	17389	17325	17101	16770	17214	16590	15580	14536	13647
4:00:23	37.0	1802	1518	2296	17349	17271	17048	16764	17242	16646	15591	14524	13651
4:05:23	37.0	1840	1540	2321	17410	17341	17000	16777	17229	16620	15586	14547	13669
4:10:23	37.0	1847	1574	2338	17462	17384	16979	16805	17149	16577	15566	14582	13640
4:15:23	37.0	1898	1596	2375	17440	17424	17084	16806	17293	16609	15595	14549	13705
4:20:23	37.0	1936	1610	2417	17427	17383	17063	16864	17320	16688	15655	14569	13691
4:25:23	37.0	1936	1628	2416	17385	17343	17120	16879	17331	16616	15604	14631	13737
4:30:23	37.0	1962	1640	2434	17524	17396	17141	16852	17205	16653	15661	14581	13689
4:35:23	37.0	1984	1677	2472	17467	17311	17166	16924	17337	16645	15646	14598	13772
4:40:23	37.0	1999	1681	2481	17456	17483	17184	16912	17322	16653	15637	14570	13832
4:45:23	37.0	2004	1703	2507	17518	17419	17184	16964	17293	16750	15673	14656	13783
4:50:23	37.0	2048	1750	2525	17611	17447	17209	17024	17392	16704	15749	14735	13761
4:55:23	37.0	2083	1765	2541	17544	17581	17174	17010	17392	16678	15678	14621	13777
5:00:23	37.0	2073	1767	2568	17494	17503	17194	17098	17425	16727	15696	14732	13851
5:05:23	37.0	2095	1778	2587	17508	17424	17190	17055	17398	16738	15730	14693	13777
5:10:23	37.0	2128	1800	2611	17612	17453	17240	17030	17458	16749	15720	14675	13884
5:15:23	37.0	2137	1827	2611	17501	17544	17232	17029	17468	16740	15701	14666	13846
5:20:23	37.0	2146	1849	2619	17541	17509	17195	17054	17389	16709	15760	14736	13842
5:25:23	37.0	2174	1865	2637	17604	17521	17232	17009	17352	16713	15717	14777	13832
5:30:23	37.0	2170	1870	2645	17549	17493	17253	17105	17420	16757	15786	14766	13875
5:35:23	37.0	2202	1872	2659	17537	17487	17250	17107	17372	16814	15710	14780	13873
5:40:23	37.0	2197	1898	2694	17594	17537	17252	17082	17479	16789	15727	14716	13892
5:45:23	37.0	2233	1899	2689	17482	17528	17209	17014	17445	16812	15731	14813	13809
5:50:23	37.0	2230	1908	2692	17547	17528	17172	17080	17430	16760	15760	14753	13893
5:55:23	37.0	2250	1935	2716	17582	17531	17258	17109	17426	16734	15766	14744	13879
6:00:23	36.9	2263	1931	2713	17568	17487	17152	17118	17396	16747	15790	14793	13863

Results

Well ID	SPL1			SPL2			SPL3			SPL4			
Well	I1	I4	I7	K1	K4	K7	M1	M4	M7	O1	O4	O7	
Max V [GFP-F-G35:485/20,528/20]	41380.000	34080.000	57500.000	831640.000	829540.000	834100.000	881840.000	866440.000	836440.000	793180.000	793720.000	740780.000	
R-Squared [GFP-F-G35:485/20,528/20]	1.000	1.000	1.000	0.999	0.999	0.999	0.998	0.999	0.999	0.999	0.999	1.000	
t at Max V [GFP-F-G35:485/20,528/20]	2:20:00	2:25:00	2:10:00	0:45:00	0:45:00	0:45:00	0:45:00	0:45:00	0:45:00	0:50:00	0:50:00	0:50:00	
Lagtime [GFP-F-G35:485/20,528/20]	1:09:45	1:15:08	1:09:17	0:31:25	0:30:52	0:30:28	0:31:53	0:31:04	0:30:29	0:34:33	0:33:58	0:33:47	
Max V [GFP-M-G100:485,528]	14480.000	11300.000	17880.000	308080.000	304480.000	305660.000	306040.000	316840.000	313000.000	296120.000	281120.000	266840.000	
R-Squared [GFP-M-G100:485,528]	0.999	0.997	0.999	0.999	0.999	0.999	0.999	0.999	0.999	0.999	0.999	0.999	
t at Max V [GFP-M-G100:485,528]	2:20:23	2:25:23	2:15:23	0:50:23	0:45:23	0:45:23	0:50:23	0:45:23	0:45:23	0:50:23	0:50:23	0:50:23	
Lagtime [GFP-M-G100:485,528]	1:18:12	1:19:39	1:11:39	0:32:21	0:31:34	0:31:04	0:33:09	0:31:41	0:31:05	0:35:15	0:34:48	0:34:23	
Count	3			3			3			3			
Mean	1:16:30			0:31:39			0:31:58			0:34:49			
Std Dev	0:04:16			0:00:39			0:01:04			0:00:26			
CV (%)	5.573			2.063			3.333			1.236			

